23S rRNA 2058A→G Alteration Mediates Ketolide Resistance in Combination with Deletion in L22
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چکیده
منابع مشابه
23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22.
Resistance to macrolides and ketolides occurs mainly via alterations in RNA moieties of the drug-binding site. Using an A2058G mutant of Mycobacterium smegmatis, additional telithromycin resistance was acquired via deletion of 15 residues from protein L22. Molecular modeling, based on the crystal structure of the large ribosomal subunit from Deinococcus radiodurans complexed with telithromycin,...
متن کاملKetolide antimicrobial activity persists after disruption of interactions with domain II of 23S rRNA.
Ketolides are the latest derivatives developed from the macrolide erythromycin to improve antimicrobial activity. All macrolides and ketolides bind to the 50S ribosomal subunit, where they come into contact with adenosine 2058 (A2058) within domain V of the 23S rRNA and block protein synthesis. An additional interaction at nucleotide A752 in the rRNA domain II is made via the synthetic carbamat...
متن کامل23S rRNA base pair 2057-2611 determines ketolide susceptibility and fitness cost of the macrolide resistance mutation 2058A-->G.
The 23S rRNA A2058G alteration mediates macrolide, lincosamide, and streptogramin B resistance in the bacterial domain and determines the selectivity of macrolide antibiotics for eubacterial ribosomes, as opposed to eukaryotic ribosomes. However, this mutation is associated with a disparate resistance phenotype: It confers high-level resistance to ketolides in mycobacteria but only marginally a...
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because we included samples from children hospitalized with or without respiratory disease. BLAST search (www.ncbi.nlm. nih.gov/blast/Blast.cgi) and phylogenetic analysis of amplicons from PCR and nested PCR indicated that samples were positive for HKU1 genotype B (samples BRA169, BRA90, and BRA99) or HKU1 genotype A (samples BRA09, BRA37, and BRA104). The sequences obtained in this study have ...
متن کاملMacrolide resistance by ribosomal mutation in clinical isolates of Streptococcus pneumoniae from the PROTEKT 1999-2000 study.
Sixteen (1.5%) of the 1,043 clinical macrolide-resistant Streptococcus pneumoniae isolates collected and analyzed in the 1999-2000 PROTEKT (Prospective Resistant Organism Tracking and Epidemiology for the Ketolide Telithromycin) study have resistance mechanisms other than rRNA methylation or efflux. We have determined the macrolide resistance mechanisms in all 16 isolates by sequencing the L4 a...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2006
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.00767-06